New: The Data Back Guide for RNA-seq results, $19
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Sample QC passed
Flow cell: 1,420 pores
Practical guides for sequencing projects

Sequencing, without the guesswork.

Short, practical guides and free tools for researchers doing sequencing for the first time. From planning the project to making sense of the data.

Find your guide →Try the free tools

Written by a molecular biologist · No coding needed · 14-day money-back guarantee

14-day money-back guarantee Instant download Secure checkout by Paddle Plain English, no coding
Our guides

Where are you in your project?

Pick your stage. Each guide is short enough to read when you need it, with spreadsheets you keep using.

NGS Project Starter Kit cover
1 · Planning

I'm planning a sequencing project

Pick the right method, design it well, pass sample QC and compare quotes on true cost.

$29NGS Project Starter Kit →
First Nanopore Run Kit cover
2 · Running it myself

I'm running my first MinION

Check your DNA, protect the flow cell, read MinKNOW and rescue a run that's going badly.

$29First Nanopore Run Kit →
Data Back Guide cover
3 · Data is back

My RNA-seq data just arrived

Check the data, read the provider's report and rerun the comparison you need, with no code.

$19The Data Back Guide →

Need two? Bundles save up to $13: Starter + Nanopore $45, Starter + Data Back $39.

Free tools

Start free

Useful on their own. No account needed; some ask for your email.

Sample QC checker

Enter your numbers and see if your sample is likely to pass.

Online tool

QC cheat sheet

One page of purity and integrity targets for DNA and RNA.

PDF

Flow cell arrival card

What to do in the first 10 minutes to protect the warranty.

PDF

Data back checklist

10 checks before you show RNA-seq results to anyone.

PDF
Guides

Free reading

Short articles on the questions people ask most.

Why sequencing samples fail QCThe common reasons, and how to avoid them→ Single-cell RNA-seq sample prepViability, clumps and timing, in pictures→ In-house MinION or outsource?The real costs, side by side→ How to read your RNA-seq results reportThe columns that matter, in plain words→

Also published openly: QC cheat sheet on Zenodo and sample checklist on protocols.io.

EA

Eric A., MSc

Molecular Biologist · Founder, SeqReady
  • MSc Molecular Biology, University of Debrecen
  • Thesis: nanopore sequencing for hospital infection control
  • Worked on the provider side of NGS services
About SeqReady

I've seen sequencing projects from both sides.

As a student, I ran nanopore sequencing to identify hospital pathogens. Later, on the provider side, I helped universities, hospitals, research labs and biotech companies plan their sequencing projects.

The same avoidable problems kept coming up: the wrong method, samples that failed QC, quotes that hid the real cost, and data nobody knew how to check. SeqReady is the set of practical guides I wish every first-time researcher had.

Questions

Good to know

Which guide should I start with?

Pick the stage you're at: planning a project (Starter Kit), running your own MinION (Nanopore Kit), or data already back (Data Back Guide).

Do I need to code?

No. Everything works with a PDF, a spreadsheet and free web tools.

What do I get, and how?

A PDF guide plus Excel / Google Sheets files. You download them right after payment.

How do I pay?

Checkout is handled by Paddle, our Merchant of Record. It accepts major cards and handles sales tax for your country.

Can I get a refund?

Yes. Ask for a full refund within 14 days of purchase. See the Refund Policy.

Is this for clinical use?

No. SeqReady guides are for research and education only.

Questions about your project?

Write to us. A real person reads every email.

[email protected]